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Human CD25/IL-2 R alpha Quantikine ELISA Kit
Human CD25/IL-2 R alpha Quantikine ELISA Kit
Human CD25/IL-2 R alpha Quantikine ELISA Kit

Human CD25/IL-2 R alpha Quantikine ELISA Kit

 詳情說(shuō)明

Human CD25/IL-2 R alpha Quantikine ELISA Kit Summary

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貨號(hào)：DR2A00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)
Sensitivity
10 pg/mL
Assay Range
78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-2 sR alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-2 sR alpha Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-2 sR alpha in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IL-2 sR alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2 sR alpha showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 sR alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 207 613 2357 188 630 2615
Standard Deviation 12.7 37.5 108 13.6 38.1 183
CV% 6.1 6.1 4.6 7.2 6 7

Recovery

The recovery of IL-2 sR alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 105 93-118
EDTA Plasma (n=5) 103 91-113
Heparin Plasma (n=5) 104 98-113
Serum (n=5) 105 99-116

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