Human Total IL-18/IL-1F4 Quantikine ELISA Kit
Human Total IL-18/IL-1F4 Quantikine ELISA Kit
Human Total IL-18/IL-1F4 Quantikine ELISA Kit Summary
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貨號(hào)：DL180
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (25 uL), Urine (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human Total IL-18.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
175
|
301
|
636
|
197
|
381
|
739
|
|
Standard Deviation
|
5.49
|
8.06
|
24.6
|
27.4
|
46.7
|
81.1
|
|
CV%
|
3.1
|
2.7
|
3.9
|
13.9
|
12.3
|
11
|
Serum, EDTA Plasma, Heparin Plasma, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
126
|
251
|
495
|
126
|
248
|
494
|
|
Standard Deviation
|
3.85
|
6.33
|
14.6
|
10.9
|
19.7
|
41.6
|
|
CV%
|
3.1
|
2.5
|
2.9
|
8.7
|
7.9
|
8.4
|
Recovery
The recovery of human Total IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
96
|
90-102
|
|
EDTA Plasma (n=4)
|
104
|
93-113
|
|
Heparin Plasma (n=4)
|
102
|
88-110
|
|
Saliva (n=4)
|
108
|
94-120
|
|
Serum (n=4)
|
100
|
96-105
|
|
Urine (n=4)
|
91
|
84-100
|
