Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Summary
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貨號：DKM100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Urine (50 uL)
Assay Range
0.2 - 10 ng/mL (Urine)
Specificity
Natural and recombinant human TIM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human TIM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIM-1 in urine. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIM-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
0.98
|
3.04
|
5.88
|
1.09
|
3.19
|
6.23
|
|
Standard Deviation
|
0.042
|
0.119
|
0.259
|
0.069
|
0.193
|
0.484
|
|
CV%
|
4.3
|
3.9
|
4.4
|
6.3
|
6
|
7.8
|
Recovery
The recovery of TIM-1 spiked to levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Urine (n=20)
|
104
|
94-112
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. For more information on linearity and hand
