Human TFF3 Quantikine ELISA Kit
Human TFF3 Quantikine ELISA Kit
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Human TFF3 Quantikine ELISA Kit Summary
貨號：DTFF30 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human Trefoil Factor 3 (TFF3)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human TFF3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TFF3 in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human TFF3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFF3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFF3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
4
|
4
|
4
|
4
|
4
|
4
|
|
Mean
|
276
|
849
|
1684
|
286
|
894
|
1712
|
|
Standard Deviation
|
6.01
|
9.13
|
37.4
|
18.3
|
44
|
95.9
|
|
CV%
|
2.2
|
1.1
|
2.2
|
6.4
|
4.9
|
5.6
|
Recovery
The recovery of human TFF3 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
Cell Culture Supernates (n=4)
|
100
|
90-114
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of human TFF3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
