Human Cyr61/CCN1 Quantikine ELISA Kit
Human Cyr61/CCN1 Quantikine ELISA Kit
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Human Cyr61/CCN1 Quantikine ELISA Kit Summary
貨號：DCYR10 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Saliva (50 uL), Human Milk (10 uL)
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk)
Specificity
Natural and recombinant human Cyr61
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Cyr61/CCN1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cyr61 in cell culture supernates, serum, plasma, saliva, and human milk. It contains CHO cell-expressed recombinant human Cyr61 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cyr61 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Cyr61.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
256
|
721
|
1442
|
249
|
712
|
1445
|
|
Standard Deviation
|
5.94
|
14.2
|
33.5
|
15.9
|
35
|
73.3
|
|
CV%
|
2.3
|
2
|
2.3
|
6.4
|
4.9
|
5.1
|
Recovery
The recovery of Cyr61 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
107
|
98-117
|
|
EDTA Plasma (n=4)
|
98
|
91-104
|
|
Serum (n=4)
|
101
|
95-113
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cyr61 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
