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Human SCF Quantikine ELISA Kit
Human SCF Quantikine ELISA Kit
Human SCF Quantikine ELISA Kit

Human SCF Quantikine ELISA Kit

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Human SCF Quantikine ELISA Kit Summary

Catalog #DCK00   

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
9 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human SCF. This assay cross-reacts 15.6% with Canine SCF, 16.1% with Feline SCF, 3.5% with Mouse SCF and 1.0% with Rat SCF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure SCF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human soluble SCF (KL-1 variant) and antibodies raised against this recombinant factor. Results obtained for naturally occurring human SCF samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human SCF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 139 655 1419 142 703 1574
Standard Deviation 5.5 12.9 54 14.6 36 94
CV% 4 2 3.8 10.3 5.1 6

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 153 709 1427 151 729 1552
Standard Deviation 3.6 11.6 61 12.1 45.8 125
CV% 2.4 1.6 4.3 8 6.3 8.1

Recovery

The recovery of SCF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 85 75-93
Citrate Plasma (n=8) 94 84-112
EDTA Plasma (n=8) 98 82-110
Heparin Plasma (n=8) 99 88-116
Serum (n=8) 99 87-111

Linearity

To assess the linearity of the assay, the following biological samples were diluted with the appropriate Calibrator Diluent and then assayed. The cell culture media was spiked with exogenous SCF.
Human SCF/c-kit Ligand ELISA Linearity

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