Human MIF Quantikine ELISA Kit
Human MIF Quantikine ELISA Kit
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Human MIF Quantikine ELISA Kit Summary
Catalog Availability
DMF00B 1 Kit / Please Inquire
SMF00B 1 Pack (6 Plates) / Please Inquire
PDMF00B 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL), Human Milk (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human MIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
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1.16
|
3.56
|
7.27
|
1.03
|
3.2
|
6.46
|
|
Standard Deviation
|
0.07
|
0.18
|
0.33
|
0.1
|
0.27
|
0.61
|
|
CV%
|
6
|
5.1
|
4.5
|
9.7
|
8.4
|
9.4
|
Recovery
The recovery of MIF spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
|
95
|
86-104
|
|
Platelet-poor EDTA Plasma (n=5)
|
95
|
80-108
|
|
Platelet-poor Heparin Plasma (n=5)
|
96
|
86-108
|
|
Serum (n=4)
|
99
|
94-104
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
