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Human VEGF-C Quantikine ELISA Kit
Human VEGF-C Quantikine ELISA Kit
Human VEGF-C Quantikine ELISA Kit

Human VEGF-C Quantikine ELISA Kit

 詳情說明


Human VEGF-C Quantikine ELISA Kit Summary


貨號(hào):DVEC00

規(guī)格：96T

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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Sensitivity
48.4 pg/mL
Assay Range
109.0 - 7,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma, Saliva)
Specificity
Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 572 1622 3243 520 1527 3069
Standard Deviation 39.3 54.4 136 50.1 102 200
CV% 6.9 3.4 4.2 9.6 6.7 6.5

Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 512 1543 3195 514 1540 3066
Standard Deviation 33.8 54.2 136 43.6 110 198
CV% 6.6 3.5 4.3 8.5 7.2 6.4

Recovery

The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 107 93-115
EDTA Plasma (n=4) 101 96-110
Heparin Plasma (n=4) 97 88-103
Serum (n=4) 99 89-106


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