熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
貨號：DTSP10
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk
The recovery of Thrombospondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL), Saliva (50 uL), Human Milk (50 uL)
Sensitivity
0.944 ng/mL
Assay Range
7.8 - 500 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Human Milk)
Specificity
Natural and recombinant human Thrombospondin-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Thrombospondin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Thrombospondin-1 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human Thrombospondin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombospondin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombospondin-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
71.9
142
282
75.8
151
294
Standard Deviation
4.34
9.56
18
4.71
8.51
17.6
CV%
6
6.7
6.4
6.2
5.6
6
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
95
86-101
Platelet-poor EDTA Plasma (n=4)
94
89-99
Platelet-poor Heparin Plasma (n=4)
93
87-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Thrombospondin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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