Human RBP4 Quantikine ELISA Kit
Human RBP4 Quantikine ELISA Kit
Human RBP4 Quantikine ELISA Kit Summary
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貨號：DRB400
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (20 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human RBP4
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human RBP4 immunoassay is a 2.5 hour solid phase ELISA designed to measure human RBP4 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human RBP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RBP4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human RBP4.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
11
|
30.3
|
61.9
|
9.8
|
27.5
|
54.7
|
|
Standard Deviation
|
0.77
|
1.73
|
5.01
|
0.84
|
1.6
|
4
|
|
CV%
|
7
|
5.7
|
8.1
|
8.6
|
5.8
|
7.3
|
Recovery
The recovery of RBP4 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
86-115
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RBP4 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
