Human PDGF-BB Quantikine ELISA Kit Summary
Human PDGF-BB Quantikine ELISA Kit Summary
Human PDGF-BB Quantikine ELISA Kit Summary
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貨號：DBB00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human PDGF-BB
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PDGF-BB in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human PDGF-BB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-BB.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
310
|
759
|
1093
|
287
|
772
|
1082
|
|
Standard Deviation
|
14
|
18.6
|
25.5
|
21.9
|
56.9
|
89.8
|
|
CV%
|
4.5
|
2.4
|
2.3
|
7.6
|
7.4
|
8.3
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
268
|
639
|
935
|
251
|
655
|
918
|
|
Standard Deviation
|
11.3
|
28.4
|
26.8
|
22.4
|
38.5
|
59.3
|
|
CV%
|
4.2
|
4.4
|
2.9
|
8.9
|
5.9
|
6.5
|
Recovery
The recovery of PDGF-BB spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
94
|
86-102
|
|
Platelet-poor EDTA Plasma (n=6)
|
101
|
88-115
|
|
Platelet-poor Heparin Plasma (n=6)
|
103
|
89-112
|
|
Serum (n=6)
|
102
|
93-114
|
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of PDGF-BB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
