Rat TNF-alpha Quantikine ELISA Kit
Rat TNF-alpha Quantikine ELISA Kit
Rat TNF-alpha Quantikine ELISA Kit Summary
貨號：RTA00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Specificity
Natural and recombinant rat TNF-alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat TNF-alpha accurately. Results obtained using natural rat TNF-alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat TNF-alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
65
|
232
|
593
|
63
|
246
|
656
|
|
Standard Deviation
|
3.3
|
5.1
|
12.5
|
6.1
|
23.6
|
57.6
|
|
CV%
|
5.1
|
2.2
|
2.1
|
9.7
|
9.6
|
8.8
|
Recovery
The recovery of rat TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
101
|
93-119
|
|
EDTA Plasma (n=5)
|
96
|
88-104
|
|
Heparin Plasma (n=4)
|
80
|
73-84
|
|
Serum (n=8)
|
93
|
83-105
|
Linearity
To assess the linearity of the assay, samples spiked with various concentrations of rat TNF-alpha were diluted with Calibrator Diluent and then assayed. Results from typical sample dilutions are shown.
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