Mouse PlGF-2 Quantikine ELISA Kit
Mouse PlGF-2 Quantikine ELISA Kit
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Mouse PlGF-2 Quantikine ELISA Kit Summary
貨號(hào)：MP200 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse PlGF-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse PlGF-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PlGF-2 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse PlGF-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PlGF-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PlGF-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
58
|
136
|
470
|
63
|
155
|
493
|
|
Standard Deviation
|
4.6
|
7.6
|
14
|
6
|
15
|
43
|
|
CV%
|
7.9
|
5.6
|
3
|
9.5
|
9.7
|
8.7
|
Recovery
The recovery of mouse PlGF-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
97
|
84-119
|
|
EDTA Plasma (n=6)
|
94
|
88-104
|
|
Serum (n=6)
|
90
|
81-102
|
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse PlGF-2 in each matrix were diluted with Calibrator Diluent and then assayed.
