Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit
Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit
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Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit Summary
貨號：DFS00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sFas
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human sFas Immunoassay is a 4.5 hour solid phase ELISA designed to measure human sFas in cell culture supernates, serum, and plasma. It contains recombinant human Fas/Fc chimera expressed from NS0 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human sFas showed dose-response curves that were parallel to the standard curves obtained using the recombinant Fas/Fc chimera. These results indicate that this kit can be used to determine relative mass values for natural human sFas.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
130
|
804
|
1424
|
140
|
811
|
1391
|
|
Standard Deviation
|
5
|
23.3
|
65.2
|
9.4
|
31.8
|
40
|
|
CV%
|
3.8
|
2.9
|
4.6
|
6.7
|
3.9
|
2.9
|
Recovery
The recovery of Fas/Fc chimera spiked to three different levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
102
|
92-107
|
|
Citrate Plasma (n=5)
|
98
|
82-116
|
|
EDTA Plasma (n=5)
|
97
|
86-111
|
|
Heparin Plasma (n=5)
|
94
|
88-109
|
|
Serum (n=5)
|
97
|
91-110
|
Linearity
To assess the linearity of the assay, five samples containing or spiked with high concentrations of sFas or Fas/Fc chimera were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
