Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
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Mouse C-Reactive Protein/CRP Quantikine ELISA Kit Summary
貨號：MCRP00 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse C-Reactive Protein/CRP immunoassay is a 4.5 hour solid-phase ELISA designed to measure CRP in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CRP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse CRP. Results obtained using natural mouse CRP showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse CRP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
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2
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3
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1
|
2
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3
|
|
n
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20
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20
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20
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20
|
20
|
20
|
|
Mean
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0.453
|
1.31
|
3.78
|
0.48
|
1.33
|
3.76
|
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Standard Deviation
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0.035
|
0.083
|
0.089
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0.052
|
0.087
|
0.314
|
|
CV%
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7.7
|
6.3
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2.4
|
10.8
|
6.5
|
8.4
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Recovery
The recovery of mouse CRP spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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95
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82-115
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CRP in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
MCRP00
