熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
貨號：PLB00B
Cell Culture Supernates, Serum, Heparin Plasma
The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (50 uL), Heparin Plasma (50 uL)
Sensitivity
13.6 pg/mL
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant porcine IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Porcine IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mature porcine IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant porcine IL-1 beta. Results obtained using natural porcine IL-1 beta show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-1 beta.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
136
348
780
141
338
783
Standard Deviation
9.8
17
40.9
12.2
20.5
31.7
CV%
7.2
4.9
5.2
8.7
6.1
4
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
106
102-109
Heparin Plasma (n=4)
111
98-117
Serum (n=4)
111
102-117
Linearity
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
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