Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Human Pentraxin 3/TSG-14 Quantikine ELISA Kit
Human Pentraxin 3/TSG-14 Quantikine ELISA Kit Summary
貨號：DPTX30
規(guī)格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (100 uL), EDTA Plasma (100 uL), Saliva (100 uL)
Assay Range
0.3 - 20 ng/mL (Serum-free Cell Culture Supern, EDTA Plasma, Saliva)
Specificity
Natural and recombinant human PTX3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human PTX3 Immunoassay is a 5.0 hour solid-phase ELISA designed to measure human PTX3 in pretreated cell culture supernates, plasma, and saliva. It contains NS0-expressed recombinant human PTX3 and has been shown to accurately quantitate the recombinant factor. Results obtained using pretreated natural human PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PTX3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum-free Cell Culture Supernates, EDTA Plasma, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
2.61
|
7.72
|
14.1
|
2.75
|
7.94
|
14.5
|
|
Standard Deviation
|
0.01
|
0.28
|
0.62
|
0.17
|
0.39
|
0.6
|
|
CV%
|
3.8
|
3.7
|
4.4
|
6.1
|
5
|
4.1
|
Recovery
The recovery of PTX3 spiked to levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
EDTA Plasma (n=4)
|
99
|
83-106
|
|
Serum-free Cell Culture Media (n=4)
|
101
|
95-108
|
Linearity
To assess the linearity of the assay, pretreated samples containing and/or spiked with high concentrations of PTX3 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
