熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
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貨號：D5000B 規(guī)格：96T
Serum, EDTA Plasma, Heparin Plasma
Cell Culture Supernates, Urine
The recovery of human IL-5 spiked to different levels throughout the range of the assay in various matrices was evaluated.
Human IL-5 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
1.08 pg/mL
Assay Range
3.9 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-5 levels in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-5 and antibodies raised against the recombinant factor and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-5.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
31.3
95.6
173
32.9
97.9
180
Standard Deviation
1.4
3.5
6.5
4
6.7
8.4
CV%
4.5
3.7
3.8
12.2
6.8
4.7
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
28.1
89.3
170
31.2
92.6
174
Standard Deviation
0.89
2
7.27
2.5
4.2
8.7
CV%
3.2
2.2
4.2
8
4.5
5
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
101
95-107
EDTA Plasma (n=4)
104
86-118
Heparin Plasma (n=4)
101
87-112
Serum (n=4)
104
88-116
Urine (n=4)
96
91-106
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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