Mouse CCL17/TARC Quantikine ELISA Kit
Mouse CCL17/TARC Quantikine ELISA Kit
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Mouse CCL17/TARC Quantikine ELISA Kit Summary
貨號：MCC170 規(guī)格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse TARC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse TARC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TARC levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TARC and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse TARC showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TARC.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
27
|
89
|
278
|
29
|
98
|
310
|
|
Standard Deviation
|
1.3
|
2.3
|
7.8
|
2.2
|
6.9
|
17.3
|
|
CV%
|
4.8
|
2.6
|
2.8
|
7.6
|
7
|
5.6
|
Recovery
The recovery of mouse TARC spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
94
|
85-113
|
|
EDTA Plasma (n=6)
|
94
|
83-109
|
|
Serum (n=6)
|
94
|
82-103
|
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse TARC in each matrix were diluted with Calibrator Diluent and assayed.
