熱門(mén)關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門(mén)關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國(guó)統(tǒng)一客服熱線：
0755-28715175
貨號(hào)：DFN00
規(guī)格：96T
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Serum, EDTA Plasma, Follicular Fluid
Cell Culture Supernates
The recovery of Follistatin spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (100 uL), EDTA Plasma (100 uL), Follicular Fluid (10 uL)
Sensitivity
83 pg/mL
Assay Range
250.0 - 16,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Follicular Fluid)
Specificity
Natural and recombinant human Follistatin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Follistatin Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human Follistatin (FS288, FS300, and FS315) in follicular fluid, cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Follistatin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human Follistatin. Results obtained using natural Follistatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Follistatin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
1996
6047
11999
1888
5604
11626
Standard Deviation
53
144
239
173
399
918
CV%
2.7
2.4
2
9.2
7.1
7.9
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
1850
5363
10734
1645
4850
10256
Standard Deviation
59
136
181
139
312
584
CV%
3.2
2.5
1.7
8.4
6.4
5.7
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
97
91-107
EDTA Plasma (n=5)
96
87-102
Serum (n=5)
95
87-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Follistatin were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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