Human IL-7 Quantikine HS ELISA Kit
Human IL-7 Quantikine HS ELISA Kit
Human IL-7 Quantikine HS ELISA Kit Summary
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貨號：HS750
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)
Assay Range
0.2 - 10 pg/mL (Cell Culture Supernates), 0.3 - 16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
0.46
|
5.79
|
11.18
|
0.51
|
6.38
|
12.95
|
|
Standard Deviation
|
0.03
|
0.19
|
0.44
|
0.06
|
0.5
|
1.3
|
|
CV%
|
6.5
|
3.3
|
3.9
|
11.8
|
7.8
|
10
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
0.5
|
1.49
|
6.86
|
0.78
|
2.13
|
8.81
|
|
Standard Deviation
|
0.04
|
0.14
|
0.61
|
0.08
|
0.16
|
0.64
|
|
CV%
|
8
|
9.4
|
8.9
|
10.3
|
7.5
|
7.3
|
Recovery
The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
103
|
89-113
|
|
Citrate Plasma (n=5)
|
100
|
92-110
|
|
EDTA Plasma (n=5)
|
112
|
83-132
|
|
Serum (n=5)
|
100
|
91-113
|
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-7 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
