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Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary
Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary
Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary

Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary

 詳情說明

Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary

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貨號：DMA00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity
10 pg/mL
Assay Range
31.2 - 1,000 pg/mL (Cell Culture Supernates), 46.9 - 1,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human MIP-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 116 442 726 53.6 313 623
Standard Deviation 3.3 5.9 14 3.7 13.4 25.7
CV% 2.8 1.3 1.9 6.9 4.3 4.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 140 688 1084 50.7 306 613
Standard Deviation 12.5 60.4 55.4 5.9 15.1 37.8
CV% 8.9 8.8 5.1 11.6 4.9 6.2

Recovery

The recovery of MIP-1 alpha spiked to different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 100 88-117
Citrate Plasma (n=5) 94 85-104
EDTA Plasma (n=5) 96 81-109
Heparin Plasma (n=5) 98 82-121
Serum (n=5) 95 87-113

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