Porcine TNF-alpha Quantikine ELISA Kit Summary
Porcine TNF-alpha Quantikine ELISA Kit Summary
Porcine TNF-alpha Quantikine ELISA Kit Summary
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貨號(hào)：PTA00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant porcine TNF-alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Porcine TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine TNF-alpha in cell culture supernates, serum, and plasma samples. It contains E. coli-expressed recombinant porcine TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure recombinant porcine TNF-alpha. Results obtained using natural porcine TNF-alpha show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine TNF-alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
79
|
260
|
646
|
88
|
279
|
679
|
|
Standard Deviation
|
4.9
|
9.2
|
27
|
8.8
|
24
|
44
|
|
CV%
|
6.2
|
3.5
|
4.2
|
10
|
8.6
|
6.5
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
104
|
358
|
880
|
106
|
348
|
866
|
|
Standard Deviation
|
7.2
|
13
|
36
|
9.7
|
32
|
72
|
|
CV%
|
6.9
|
3.6
|
4.1
|
9.2
|
9.2
|
8.3
|
Recovery
The recovery of porcine TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
99
|
91-105
|
|
EDTA Plasma (n=6)
|
98
|
83-111
|
|
Heparin Plasma (n=4)
|
91
|
82-99
|
|
Serum (n=6)
|
105
|
91-120
|
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with various concentrations of porcine TNF-alpha in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
