Mouse/Rat CCL5/RANTES Quantikine ELISA Kit
Mouse/Rat CCL5/RANTES Quantikine ELISA Kit
Mouse/Rat CCL5/RANTES Quantikine ELISA Kit Summary
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貨號：MMR00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Citrate Plasma (25 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant mouse and rat RANTES. This assay cross-reacts 1.3% with recombinant Human RANTES.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat CCL5/RANTES Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat RANTES levels in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse RANTES as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant RANTES. Results obtained using natural mouse or rat RANTES showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat RANTES.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Citrate Plasma, Platelet-poor Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
18.8
|
104
|
198
|
20.1
|
109
|
208
|
|
Standard Deviation
|
0.7
|
1.9
|
4.1
|
1.6
|
5.6
|
11.5
|
|
CV%
|
3.7
|
1.8
|
2.1
|
8
|
5.1
|
5.5
|
Recovery
The recovery of RANTES spiked to three levels in mouse samples throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=8)
|
108
|
98-115
|
|
Platelet-poor Citrate Plasma (n=5)
|
102
|
95-110
|
|
Platelet-poor EDTA Plasma (n=5)
|
99
|
86-110
|
|
Serum (n=5)
|
97
|
90-107
|
Linearity
To assess the linearity of the assay, four or more samples containing or spiked with various concentrations of RANTES in each matrix were diluted with Calibrator Diluent and then assayed.
