Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary
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貨號：DBLYS0B
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human BAFF/BLyS
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF/BLyS and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
433
|
1000
|
2343
|
474
|
1118
|
2111
|
|
Standard Deviation
|
28.2
|
72
|
78.6
|
46.8
|
112
|
244
|
|
CV%
|
6.5
|
7.2
|
3.4
|
9.9
|
10
|
11.6
|
Recovery
The recovery of BAFF/BLyS spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
96
|
90-103
|
|
EDTA Plasma (n=4)
|
94
|
84-101
|
|
Heparin Plasma (n=4)
|
94
|
89-107
|
|
Serum (n=4)
|
93
|
84-106
|
Linearity
