Human PlGF Quantikine ELISA Kit
Human PlGF Quantikine ELISA Kit
Human PlGF Quantikine ELISA Kit Summary
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貨號：DPG00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human PlGF
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human PlGF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human PlGF in cell culture supernates, serum, plasma, and urine. The immunoassay kit contains E. coli-expressed recombinant human PlGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human PlGF accurately. Results obtained using natural human PlGF showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PlGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
40.9
|
138
|
541
|
46.9
|
149
|
555
|
|
Standard Deviation
|
2.4
|
7.3
|
38.2
|
6.4
|
13.5
|
32.9
|
|
CV%
|
5.9
|
5.3
|
7.1
|
13.6
|
9.1
|
5.9
|
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
54.3
|
175
|
658
|
55
|
184
|
724
|
|
Standard Deviation
|
3.8
|
6.3
|
36.9
|
6.5
|
20.3
|
78.9
|
|
CV%
|
7
|
3.6
|
5.6
|
11.8
|
11
|
10.9
|
Recovery
The recovery of PlGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
97
|
82-107
|
|
Citrate Plasma (n=5)
|
99
|
93-120
|
|
EDTA Plasma (n=5)
|
102
|
92-117
|
|
Heparin Plasma (n=5)
|
94
|
81-113
|
|
Serum (n=5)
|
96
|
88-118
|
|
Urine (n=4)
|
97
|
85-112
|
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of PlGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
