Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit
Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
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貨號：RLB00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Specificity
Natural and recombinant rat IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 beta in cell culture supernates, serum, and EDTA plasma. It contains recombinant rat IL-1 beta and antibodies raised against recombinant rat IL-1 beta. This immunoassay has been shown to quantitate the recombinant rat factor accurately.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
83.4
|
253
|
610
|
82.3
|
249
|
626
|
|
Standard Deviation
|
7.3
|
9.9
|
24
|
4.7
|
10.3
|
27
|
|
CV%
|
8.8
|
3.9
|
3.9
|
5.7
|
4.1
|
4.4
|
Recovery
The recovery of rat IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=5)
|
100
|
89-110
|
|
EDTA Plasma (n=5)
|
98
|
89-111
|
|
Serum (n=5)
|
108
|
97-120
|
Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
