Human Lipocalin-2/NGAL Quantikine ELISA Kit
Human Lipocalin-2/NGAL Quantikine ELISA Kit
Human Lipocalin-2/NGAL Quantikine ELISA Kit Summary
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貨號：DLCN20
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human Lipocalin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Lipocalin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lipocalin-2 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Lipocalin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lipocalin-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
1.14
|
3.45
|
7.54
|
1.05
|
3.37
|
6.92
|
|
Standard Deviation
|
0.041
|
0.107
|
0.334
|
0.083
|
0.204
|
0.387
|
|
CV%
|
3.6
|
3.1
|
4.4
|
7.9
|
6.1
|
5.6
|
Recovery
The recovery of Lipocalin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
93
|
88-101
|
|
Urine (n=4)
|
90
|
84-97
|
