Mouse IL-10 Quantikine ELISA Kit
Mouse IL-10 Quantikine ELISA Kit
Mouse IL-10 Quantikine ELISA Kit Summary
Catalog Size / Price
M1000B 1 Kit (2 Plates) / Please Inquire
SM1000B 1 Pack (6 Plates) / Please Inquire
PM1000B 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-10 accurately. Results obtained using natural mouse IL-10 showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-10.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to
assess inter-assay precision over two lots of kit components. Assays were
performed by at least three technicians
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
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76.3
|
123
|
815
|
71.4
|
115
|
809
|
|
Standard Deviation
|
2.56
|
2.85
|
17
|
5.39
|
7.64
|
55.5
|
|
CV%
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3.4
|
2.3
|
2.1
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7.5
|
6.6
|
6.9
|
Recovery
The recovery of IL-10 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
|
Cell Culture Samples (n=4)
|
99
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95-103
|
|
EDTA Plasma (n=4)
|
91
|
86-99
|
|
Heparin Plasma (n=4)
|
89
|
82-98
|
|
Serum (n=4)
|
89
|
85-96
|
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