Mouse IFN-gamma Quantikine ELISA Kit
Mouse IFN-gamma Quantikine ELISA Kit
Mouse IFN-gamma Quantikine ELISA Kit Summary
Catalog # Availability Size / Price
MIF00 1 Kit (2 Plates) / Please Inquire
SMIF00 1 Pack (6 Plates) / Please Inquire
PMIF00 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Assay Range
9.4 - 600 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IFN-gamma
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gamma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
45.1
|
143
|
528
|
42.8
|
139
|
518
|
|
Standard Deviation
|
2.1
|
4.4
|
11.8
|
3.6
|
13.6
|
46.5
|
|
CV%
|
4.7
|
3.1
|
2.2
|
8.4
|
9.8
|
9
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
44.8
|
156
|
518
|
43.3
|
144
|
509
|
|
Standard Deviation
|
2.2
|
5
|
14.2
|
3.6
|
14.7
|
48.6
|
|
CV%
|
4.9
|
3.2
|
2.7
|
8.3
|
10.2
|
9.5
|
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