中文名稱：拮抗凋亡轉(zhuǎn)錄因子抗體

產(chǎn)品編號：ZIKER-1229R
英文名稱：AATF
別    名：Apoptosis antagonizing transcription factor; CHE1; DED; AATF; AATF_HUMAN; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; Protein AATF;Rb binding protein Che 1.  
規(guī)    格 ：100ul  200ul
研究領(lǐng)域：腫瘤  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來源：Rabbit
克隆類型：Polyclonal
交叉反應(yīng)： Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, 
產(chǎn)品應(yīng)用：ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量：63kDa
細(xì)胞定位：細(xì)胞核 
性    狀：Lyophilized or Liquid
濃    度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived from human AATF:451-560/560 
亞    型：IgG
純化方法：affinity purified by Protein A
儲 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
產(chǎn)品介紹background:
The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Function:
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.

Subunit:
Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.

Subcellular Location:
Nucleus, nucleolus.

Tissue Specificity:
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.

Post-translational modifications:
Hyperphosphorylated during the G1/S phase transition.

Similarity:
Belongs to the AATF family.

SWISS:
Q9NY61

Gene ID:
26574

Database links:
Entrez Gene: 26574 Human

Entrez Gene: 56321 Mouse

Omim: 608463 Human

SwissProt: Q9NY61 Human

SwissProt: Q3URN5 Mouse

SwissProt: Q9JKX4 Mouse

Unigene: 195740 Human

Unigene: 257482 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

AATF屬于細(xì)胞凋亡的拮抗轉(zhuǎn)錄因子,有抑制細(xì)胞凋亡的發(fā)展過程。